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Microbiological water analysis is a method of analyzing water to estimate the numbers of bacteria present and to allow for the recovery of microorganisms in order to identify them. The method of examination is the plate count. The plate count method relies on bacteria growing a colony on a nutrient medium, so that the colony becomes visible to the naked eye, and the number of colonies on a plate can be counted. Most laboratories use a method, whereby sample volumes of 100 mL (or greater) are vacuum filtered through purpose-made membrane filters, and these filters are themselves laid on nutrient medium within sealed plates [18]. A nonselective medium is used to obtain a total enumeration of the sample (called a heterotrophic plate count). When it is desirable to obtain a specific bacterial species, a selective medium can be used.